2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2?

Question

Ernest Larson

Chemistry

21 March, 18:37

2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2?

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Answers (1)

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Klein · Answer 1

Tube 2 has a total dilution of 1:50

Explanation:

We have a 2 ml serum sample added to a 18 mL phosphate buffered saline sample in tube 1. This means now in tube 1 there is 20 mL.

We have a 1:10 ( = 2:20) dilution here.

10 ml of this 1:10 diluted tube 1 is taken and added to a 40 mL of PBS in tube 2.

Now we have 50 mL in tube 2.

This is a 10:50 ( = 1:5) dilution.

The total dilution is 10x5 = 50

So the total ditultion has a rate 1:50

Tube 2 has a total dilution of 1:50