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8 March, 11:34

How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 0.2 mL? 1 microliter is 10-6 L or 10-3 mL.

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  1. 8 March, 11:36
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    The required volume of the original sample required is 2 micro liter

    Explanation:

    assuming the original sample concentration is 1N

    after final dilution of 10-2 solution concentration becomes 0.01 N

    normality of original sample = 1 N

    normality of final solution = 0.01 N

    volume of original sample=?

    volume of final solution = 0.2 mL

    Considering thef formula below:

    N1V1 = N2V2

    V1 = (N2V2) / N1

    = (0.01*0.2) / 1

    = 0.002 mL

    1 milli liter = 1000 micro liter

    0.002 mL = 2 micro liter

    The original sample required is 2 micro liter
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