During the GFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M. W. = 40g/mol), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.

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Sexton

Chemistry

6 August, 20:54

During the GFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M. W. = 40g/mol), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.

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Amanda Barr · Answer 1

150 mM NaCl = 0.15 M NaCl

500 ml of 0.15 M NaCl = 500 x 0.15 ml of M NaCl

= 75 ml of M NaCl

M. W = 40 g

1000 ml of M NaCl requires 40 g

75 ml of M NaCl requires (40/1000) x75

= 3 g

We shall take 500 ml of water in a graduated cylinder. Then we weigh 3 g of salt and add it to 500 ml of water and mix it. We shall get 500 ml of 150mM NaCl.