Peptides can be separated according to their charges via an ion-exchange column. in a cation-exchange column at neutral ph, which peptide would elute last?

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Anderson Branch

Chemistry

13 March, 10:55

Peptides can be separated according to their charges via an ion-exchange column. in a cation-exchange column at neutral ph, which peptide would elute last?

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Emily · Answer 1

A cation-exchange column is a type of chromatography that separates substances according to their charges. The cation-exchange column in particular has a negatively charged resin that will attract positively charged ions.

Applying this principle with amino acids, we can say, for example that the negatively charged ones (e. g., aspartate and glutamate) will elute first compared to the positively charged ones (e. g., lysine, arginine, and histidine), which will elute last.