4. You are preparing 250 mL of gel mix for protein electrophoresis, which requires a final concentration of 1.6% SDS. The lab has a 10% stock solution of SDS. How much SDS stock solution (in mL) do you use? Round your answer to the nearest whole number. (Ch. 2.1)

Question

Keyon Coleman

Chemistry

5 September, 07:57

4. You are preparing 250 mL of gel mix for protein electrophoresis, which requires a final concentration of 1.6% SDS. The lab has a 10% stock solution of SDS. How much SDS stock solution (in mL) do you use? Round your answer to the nearest whole number. (Ch. 2.1)

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Answers (1)

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Mercedes Harvey · Answer 1

40 mL

Explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2

Where C is the concentration, V the volume, 1 is the initial solution and 2 the final solution. So, the final volume will be 250 mL, with 1.6% of concentration:

10xV1 = 1.6x250

V1 = 40 mL

So, it's necessary 40 mL of the SDS stock solution.