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24 August, 12:25

The absorbance of a chlorophyll a standard solution in ethanol (density = 0.785 g/mL) is measured in

a 1.3 cm cuvette or path length. The absorbance is 0.765 and the molar absorptivity coefficient is 86,300 M-1cm-1.

The molecular weight for chlorophyll a is 893.49 g/mol.

What is the concentration of the solution in ppm?

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Answers (1)
  1. 24 August, 12:29
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    Let A = ebc

    0.765 = 86,300*1.3*c

    Solve for c = approximately 7E-6 Molar

    = mols/L soln.

    g = 7E-6*893.49

    = about 0.006 g chlorophyll/L soln.

    1000 x 0.785 = 785 g ethanol.

    Conc. = about 0.006g chlorophyll/785 g soln.

    Change that to ppm. by using formula:

    (0.006/785) * 1E6
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