An assay protocol requires you to add 12.5 mg of lysozyme to 1.0 L of buffer. You have a lysozyme stock vial labeled 25 mg/mL. How many microliters (UL) of the stock will you need to add to your solution.

Question

Conrad Harrison

Chemistry

3 December, 21:00

An assay protocol requires you to add 12.5 mg of lysozyme to 1.0 L of buffer. You have a lysozyme stock vial labeled 25 mg/mL. How many microliters (UL) of the stock will you need to add to your solution.

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Answers (1)

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Ishaan Barr · Answer 1

500 uL

Explanation:

Our stock is 25 mg/mL. This means for every mL (1000 uL) there are 25 mg of lysozyme, and we need 12.5 mg.

So 25 / 1000 uL = 12.5 / X

X = (12.5 mg x 1000 uL) / 25

X = 500 uL

We need to add to our solution 500 uL of the lysozyme stock.