In "The Negro Speaks of Rivers," Hughes' description of the Mississippi River is symbolic of A. Lincoln's role in the Civil War. B. African Americans' newfound freedom. C. Lincoln's memorable trip to New Orleans. D. African Americans overcoming adversity.

The answer is option D: African Americans overcoming adversity.

In the poem "The Negro Speaks of Rivers," the author Langston Hughes describes the Mississippi River from a train and contemplates its significance during slavery times. He also refects on his own person, his ancestors and the difficulties and injusticies they have been through.