What overall reason for the colonies to separate from Britain does Jefferson outline in the second paragraph

The reason outlined by Jefferson in the second paragraph behind the separation of colonies from Britain was the violation of certain rights like the pursuit of happiness, right to life and liberty. These rights were never given importance by the British Empire and this led to the revolt by the colonists. As per Jefferson the government failed to protect these rights for the people and hence the colonists had the full right to overthrow the tyrannically British government and create a government of their own.