Which of the following facts about Jamestown's location caused hardship for its settlers? It was on an island in the Chesapeake Bay. It was inland near a swampy area of a river. It was on the shore of Chesapeake Bay. It was on the shore of the Atlantic Ocean.

The answer is the second option, that is - It was inland near a swampy area of a river

Jamestown's was located inland near a swampy area of river that contained mosquitoes that carried malaria. This disease killed many settlers. Not only this, but the water they drank from the river was also contaminated with germs. These factors caused a lot of hardships to its settlers.