The constitution's creation of a national government with separation of powers is based from what amendment?

The separation of powers is based on the fourth amendment.

Explanation:

The fourth amendment to the U. S constitution provides a criterion for establishing the separation of powers between the executive, legislative and judiciary branch, namely, that the government cannot intervene on an individuals life on unreasonable terms. This amendment protects each individual from the abuse of government's power through unreasonable search and seizure of property. The law regulates the government's actions, and the judicial branch limits the power of government to act on an individual on unreasonable terms.