You put a culture of cells on the shaker at 37°C. You had intended to come into lab the next day to harvest the cells and purify plasmid DNA from them. Instead, you wake up the next day with the flu. You are home for 2 days with a fever and chills. When you drag yourself back into lab, 3 days after you put the culture on the shaker, you check your cells. You have a dense growth and an OD600 greater than 1.0. Should you attempt to purify the plasmid DNA from this culture?
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Home » Biology » You put a culture of cells on the shaker at 37°C. You had intended to come into lab the next day to harvest the cells and purify plasmid DNA from them. Instead, you wake up the next day with the flu. You are home for 2 days with a fever and chills.