A biologist wants to use a radioactive isotope of nitrogen to radiolabel certain molecules in a culture of cells she is growing. If the biologist incubates her cells with N-15 (a heavy isotope of nitrogen), which group of macromolecules will be labeled in the culture of cells? A) glucose B) glycogen C) lipids D) proteins

Question

Hudson Buckley

Biology

20 October, 03:57

A biologist wants to use a radioactive isotope of nitrogen to radiolabel certain molecules in a culture of cells she is growing. If the biologist incubates her cells with N-15 (a heavy isotope of nitrogen), which group of macromolecules will be labeled in the culture of cells? A) glucose B) glycogen C) lipids D) proteins

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Daniel · Answer 1

D) proteins

Explanation:

The N-15 is one of the isotopes used in proteomics, in which the full set of proteins expressed by a genome are studied. The method is called Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) in which the labeled amino acids are used to estimate the protein levels. With this method, the diseases biomarkers can be identified and studied.