A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme, but it also occasionally makes mistakes and cuts elsewhere. Would this type of enzyme be a useful enzyme to use in molecular cloning?

Question

Azul Estrada

Biology

19 March, 18:38

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme, but it also occasionally makes mistakes and cuts elsewhere. Would this type of enzyme be a useful enzyme to use in molecular cloning?

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Answers (1)

Know the Answer?

Santino Strong · Answer 1

No, in molecular cloning cutting mistakes can lead to in correct ligation

C) No, because cutting mistakes could lead to incorrect cloning.

Explanation:

in molecular cloning, if you want to insert a specific gene of interest in to specific vector, the selection of enzyme and strategy before that is very important. The restriction enzyme should be selected which restrict at the desired location in the cloning sites. Any mistake in these selection or undesirable mutation mistakes can lead to in correct or unsuccessful cloning results.