A protein subunit from an enzyme is part of a research study and needs to be characterized. a total of 0.185 g of this subunit was dissolved in enough water to produce 2.00 ml of solution. at 28? c the osmotic pressure produced by the solution was 0.138 atm. what is the molar mass of the protein?

Question

Bradley Moody

Chemistry

23 July, 14:59

A protein subunit from an enzyme is part of a research study and needs to be characterized. a total of 0.185 g of this subunit was dissolved in enough water to produce 2.00 ml of solution. at 28? c the osmotic pressure produced by the solution was 0.138 atm. what is the molar mass of the protein?

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Krystal Velazquez · Answer 1

The osmotic pressure of a solution is calculated from the product of concentration in molarity, temperature and the universal gas constant, π = CRT. To determine the molar mass, we first calculate the concentration as follows:

π = CRT

0.138 = C (0.08205) (28 + 273.15)

C = 5.5849x10^-3 mol / L

We then multiply the volume of the solution to obtain the amount in moles.

moles protein = 5.5849x10^-3 mol / L (.002 L) = 1.12x10^-5 mol

Molar mass = 0.185 g / 1.12x10^-5 mol = 16562.432 g/mol