Calculate the volume of compound Y needed to make a 350 pg/ml treatment solution in 2 ml of 1x PBS using a stock solution containing 50 ml of compound Y at 50 pg/ml. 14 l Not possible to make as described 140 pl 1.4 mi

14 mL

Explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2, where C is the concentration, V is the volume, 1 is the initial solution and 2 the final solution.

The final solution must have 2 mL and a concentration of 350 pg/mL, and the initial solution has a concentration of 50 pg/mL.

Then:

50xV1 = 350x2

50xV1 = 700

V1 = 700/50

V1 = 14 mL