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12 March, 15:05

Consider that you have a 100 mM stock solution and you need to prepare 10 mL of a 30 mM solution. How many milliliters of the stock solution do you need? mL How many milliliters of deionized water do you need? mL

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  1. 12 March, 15:30
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    V₁ = 3.0 mL

    V (H₂O) = 7 mL

    Explanation:

    Given data

    Initial concentration (C₁) : 100 mM Initial volume (V₁) : ? Final concentration (C₂) : 30 mM Final volume (V₂) : 10 mL

    In order to determine the volume of the stock solution, we will use the dilution rule.

    C₁ * V₁ = C₂ * V₂

    100 mM * V₁ = 30 mM * 10 mL

    V₁ = 3.0 mL

    The volume of deionized water required is:

    V (H₂O) = 10 mL - 3.0 mL = 7 mL
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